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Fig. EV6 Beneficial effect of the Hits upon treatment at symptomatic stage A. Phenotype of gan MO-injected embryos at 48 hpf, with deficits in touch-responsiveness and impairment of axons and neuromuscular junctions (znp1: green; αBTX: α-bungarotoxin: red). Scale bar represents a length of 100 μm. B–E. Restoration of motility of 5-day-old gan larvae, when treated from 48 hpf with Aceclidine Hydrochloride (Acecl.) (B), Oxymetazoline Hydrochloride (Oxymet.) (C), Trichlormethiazide (Trichlor.) (D) and Phentolamine Hydrochloride (Phento.) (E). Hits were applied from 5 to 30 μM concentrations, with daily bath changes; data are not provided for the higher doses (20 and 30 μM) due to toxicity. Results show the distance traveled during 1 h by gan MO-injected larvae, treated with either DMSO or Hits at 5 and 10 μM. In the absence of normality of distribution of the data, a nonparametric Kruskal–Wallis test is applied; means ± SEM are represented. **P ≤ 0.01.