Fig. 4

Fig. 4

Comparative transcriptomic analysis of cones in WT-P23H integrated dataset reveals changes in metabolism and circadian regulation. A Violin plots showing levels of expression of short- and long-wavelength opsins in cone clusters 2 and 6. B Dot plot showing the expression level and the percentage of cells expressing DEGs involved in different metabolic functional pathways between WT and P23H. C Dot plot showing the expression level and the percentage of cells expressing DEGs involved in circadian regulation between WT and P23H. D Hiplex fluorescent in situ hybridization for tefa, per1, and per2 in sections of adult retina. The fluorescent product is shown in the left third of each image, while the remainder displays a binary mask in the fluorescent in situ hybridization product channel. All three genes show increased expression throughout the retina in P23H compared to WT, reflecting the changes captured by single-cell transcriptome analysis. Scale bar applies to all panels