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Fig. 2 The experimental workflow chart. Larvae at 24 hpf were dechorionated and co-exposed to 70 μM MPTP and three different concentrations of C-PC peptides (12.5, 25, and 50 μg mL−1, respectively). The analyses of the dopaminergic neurons, neural vasculature, and ROS content were conducted at 96 hpf. The levels of oxidative stress indicators, apoptosis, and acetylcholinesterase (AChE) activity were detected at 120 hpf. At 144 hpf, the treated zebrafish larvae were subjected to evaluations of locomotor behavior and expressions of genes associated with oxidative stress, autophagy, and apoptosis.
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