Fig. 1
The relationship between temperature and swimming activity of zebrafish. A box plot was used to illustrate the distances moved by zebrafish in each experimental group. Black dots indicate outliers. Triangle markers represent the average distances moved.
A. Changes in swimming activity in response to temperature variations. Voluntary swimming activity decreases with lower temperatures but increases as temperatures rise. Asterisks indicate significant differences compared to swimming activities at the initial water temperature of 25 °C (vs 25 d1-3; *: p < 0.05, **: p < 0.01, ***: p < 0.001).
B. Swimming activity at different temperatures. A significant difference in distance moved was observed between temperatures of 20 °C and 15 °C. In the post-hoc test, asterisks are used to indicate statistical significance in comparisons between swimming activities at different temperatures (***: p < 0.001).