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Fig. 3 Example of in toto videomicroscopy of axons and macrophages. A–F. Still images from a high-resolution time-lapse recording of a macrophage (green) and a lateralis sensory nerve (red) in the trunk of a larval zebrafish. A single macrophage is seen migrating towards the nerve immediately after it has been severed with a laser. A second macrophage is seen below, which never makes contact with the nerve. Over time, the macrophage moves along the nerve, surveilling damage (which can be seen as a discontinuity of the nerve on the left-hand side of the panels (C and D). The drawings below each panel (dotted lines) describe the two-dimensional shape changes ofthis single macrophage during the course of videomicroscopic imaging. Scale bars is 50 μm.