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Fig. 3 sgANXA5/A6 MDA-MB-231 cells exhibit morphology and growth properties similar to control cells. (a) ANXA5/A6 deficient MDA-MB-231 cells were established by the CRIPSR-Cas9 strategy. The cellular content of ANXA5 and ANXA6 in sgANXA5/A6 MDA-MB-231 cells was quantified by western-blotting. The expression of ANXA5 and ANXA6 is decreased of more than 95% and 85% in sgANXA5/A6 MDA-MB-231 cells, respectively. (b) Control and sgANXA5/A6 MDA-MB-231 cells, which expressed constitutively the tdTomato fluorescent protein (red), were imaged by fluorescence microscopy. Scale bars = 40 μm. (c) Proliferation of control or sgANXA5/A6 MDA-MB-231 cells was monitored with a lensfree microscope enabling long-term observation of a large cell population (Allier et al., 2017). The averaged number of cells (+/− SEM) normalized to the number of seeded cells from three independent experiments is presented.