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Fig. 2 Invasiveness properties in breast cancer cells are correlated with high expression of ANXA5 and ANXA6. 10 ?g of protein extracts from MDA-MB-231 and MCF7 cells were analyzed by western-blotting. (a) Representative image of western-blot analysis showing the revelation of ANXA5 and ANXA6 in MDA-MB-231 and MCF7 cells, compared to GAPDH (loading control). The whole membranes are presented in Figure S2. (b) The histogram presents mean values (± SEM) of the ratio ANX/GAPDH from five independent experiments, analyzed by the gel analysis plugging of ImageJ. Student t-test for independent samples. **p < 0.01.