Excess carbohydrates promoted lipogenesis in zebrafish and ZFL (means ± SEM, n = 6). C0, C10, C20, C30, C40, and C50 represent diets in which dextrin levels are 0%, 10%, 20%, 30%, 40%, and 50%, respectively. L represents a linear trend, Q represents a quadratic trend, C represents a cubic trend, and # represents the significant of the corresponding regressions. HG represents high glucose. (a–d) Lipogenesis-related genes' expression in zebrafish liver, (e) representative images of SREBP-1c immunofluorescence staining in ZFL, scale bar, 5 μm, (f) SREBP-1c was quantified by mean green fluorescence intensity by immunofluorescence staining in ZFL, and (g) lipogenesis-related genes' expression in ZFL. Labeled means without a common letter differ among C0, C10, C20, C30, C40, and C50, P < 0.05 (one-way ANOVA, Duncan's post hoc test). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.005 (two-tailed independent t-test).
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