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Fig. 1
Fly arena design. a. Mechanical assembly of OptoPi using CAD software on the right. The stimulation and illumination platforms that surround the arena move freely and independently. Fully assembled arena on the left with the Raspberry Pi HQ camera visible in the centre. b. The Raspberry Pi both controls the camera, which records the experiment, and communicates via serial port with the Arduino Mega 2560. The Arduino Mega 2560 has its own shield to allow the researcher to control the source, position and intensity of light as well as the camera position. c. Examples of different lighting configurations. Ambient lighting works best for dark animals such as adult flies and larvae zebrafish when no optogenetic manipulation is required. For optogenetic manipulation and transparent animals such as fly larvae, IR lighting is preferred.