Levels of the downstream transcription factor mRNAs after knockdowns of (A) let-7b and (B) mir-223. Quantitative real-time PCR showing the fold change of the transcriptional factor gene expression in daily wild-type (WT) controls (blue bars) and knockdown (KD) samples (grey bars). Four replicates containing six injected fish were used for each replicate sample and control experiments (N = 4). The lines on the top of the bars represent a significant difference between WT and knockdown samples. Error bars represent mean ± SD. *, **, ***, **** and ns (non-significant) represent p ≤ 0.05, p ≤ 0.01, p ≤ 0.001, p ≤ 0.0001, p >0.05 respectively.
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