ZFIN Image: El Maï et al., 2023, Fig. 4

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Fig. 4

Gut-specific tert expression rescues systemic tissue degeneration.

Expression of telomerase in the gut of tert mutant fish rescued tissue degeneration in the testes, visceral adipose tissue, muscle and eye. a, Representative image of a longitudinal section of a zebrafish stained with H&E. The locations of each tissue analyzed in the study are indicated by arrows. b, Representative images of testes, kidney, visceral adipose tissue, subcutaneous adipose tissue, muscle and eye from 9-month-old fish stained with H&E (right). Except for the kidney, histological quantifications were performed for each tissue (left), namely the mature spermatids area (n_WT = 10 fish, 𝑛𝑡𝑒𝑟𝑡−/−noCre $n_{t e r t^{- / -} no Cre}$ = 8 fish and 𝑛𝑡𝑒𝑟𝑡−/−+Cre $n_{t e r t^{- / -} + Cre}$ = 9 fish), adipocyte area (n_WT = 9 fish, 𝑛𝑡𝑒𝑟𝑡−/−noCre $n_{t e r t^{- / -} no Cre}$ = 9 fish and 𝑛𝑡𝑒𝑟𝑡−/−+Cre $n_{t e r t^{- / -} + Cre}$ = 9 fish), muscle fiber thickness (n = 8 fish per group), retinal pigmented epithelium (RPE) and photoreceptor layer (PRL) (n_WT = 7 fish, 𝑛𝑡𝑒𝑟𝑡−/−noCre $n_{t e r t^{- / -} no Cre}$ = 8 fish and 𝑛𝑡𝑒𝑟𝑡−/−+Cre $n_{t e r t^{- / -} + Cre}$ = 8 fish), respectively. Scale bar, 20 µm. All data are presented as the mean ± s.e.m.; *P < 0.05; **P < 0.01, ***P < 0.001, using a one-way ANOVA and post hoc Tukey test; *P < 0.05, using a Kruskal–Wallis test and post hoc Dunn test.

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