Fig. 3
Both IAHCs and SBCs are depleted in Gata2+/? AGMs. (A) Illustration of the AGM region dissected for analysis of E11 WT or Gata2+/? embryos. Representative images of CD31+cKit+ cells obtained by confocal imaging of E11 WT AGM (B), Gata2+/? AGM (C), IAHCs (D), SBC (E), and aortic lumen (AL) (F) cell. Scale bars, 50 ?m. (G) Quantification of CD31+cKit+ cells located in IAHCs, as SBCs, and AL cell within E11 WT (n = 3) and Gata2+/? (n = 4) AGMs. (H) FPKM values of CD27 depicted for each stage of the maturation and compared between WT and Gata2+/?. Error bars represent standard error of the mean. ?P < .05, ??P < .01.