Fig. 1

Fig. 1 Fig. 1. Exposure and analysis timeline. Fertilised eggs were collected at ∼2 h post-fertilization (hpf) and allowed to develop up to 24 hpf. At this point, the chorion was removed by pronase treatment and at 48 hpf animals were exposed for 72 h to 250 μM 6-OHDA, concomitantly to 24-EPI at different concentrations (0.01, 0.1 and 1 μM) and to 1 μM 24-EPI. Throughout the experimental period, developmental effects were assayed and, at the end of the exposure period, behavioural, oxidative-stress related parameters and TH-immunofluorescent neurons were evaluated.