Figure 6—figure supplement 1.

Figure 6—figure supplement 1. Excess Pdgfaa expression led to precocious dermal stratification in wild-type and eda mutant fish.

(A) Pdgfaa expression (magenta), stringently selected for basal epidermal expression, caused precocious dermal stratification, labeled with ET37:EGF (green) in wild-type and eda mutants (n = 8 clones in four fish for wild-type; 9 clones in four fish for eda mutant). Arrows indicate regions of precocious dermal stratification. Arrowheads indicate regions where normal dermal stratification is occurring. (B) Pdgfaa expression did not lead to dysmorphic and mis-patterned scales, visualized with sp7:EGFP (green) in wild-type and did not rescue scale formation in eda mutants (n = 54 clones in 8 wild-type fish, 70 clones in 10 eda mutant fish). Scale bars, 50 μm (A, B). (C) Differentially expressed signaling ligands downregulated in hypoTH basal epidermal cells.