Figure 2—figure supplement 1.

Figure 2—figure supplement 1. In situ hybridization using probes against specific transcripts localizes heterogeneous scale-forming cell (SFC) in the developing scale.

(A) Live imaging of differentiated SFC expressing sp7:nEOS (green) surrounded by a halo of pre-SFC labeled with ET37:EGFP (magenta). (B) UMAP visualization of sp7+ differentiated SFC and sp7- pre-SFC. (C) UMAP visualization of lef1 and axin2, transcripts localized to pre-SFC and margin SFC in previously published in situ hybridization (Aman et al., 2018). (D, E) itga5 and tcf7 were expressed in pre-SFC and SFC at the scale margin. (F) col10a1a was highly expressed in scale margin SFCs and radii SFCs but not pre-SFCs. (G, H) itgb3b and jag1a localized to radii SFCs. (I, J) col10a1b and chad were expressed predominantly in SFC at the scale focus. Scale bars, 50 μm.