Figure 2

Figure 2

Effects of neu3.2 morpholino injection on zebrafish development. Representative images of the phenotype at 24 and 72 hpf obtained after the injection of neu3.2-MO. The injected embryos were compared with embryos injected with the standard morpholino (STD-MO). The embryos were injected with 1 pmol/embryo of neu3.2-splicing morpholino at 1–2 cell stages. For each injection experiment (n = 5), about 150 embryos were analyzed for morphology and classified with mild and severe phenotypes. The graphic in the bottom right indicates the percentage of embryos with mild and severe phenotypes. The results are expressed as mean ± SD of 5 independent experiments. (**** p < 0.0001 vs. injected embryos with standard morpholino-STD-MO—Unpaired; two-tailed t-test).