ZFIN Image: Zizioli et al., 2023, Figure 1

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Figure 1

Soluble sialidase activity in zebrafish embryo and adult organs. Total extracts from wild-type embryos (A) and organs of 9-month-old adult fish (B) were assayed for soluble sialidase activity, as described in the Section 4 (n = 30). Extracts from various embryonic stages (12, 16, 24, 48, 72, 96 and 120 hpf) and from ovary, brain, heart and muscle were used as the enzyme sources. Assays were performed with up to 10 µg of total proteins. Sialidase activity was measured using 4-MU-NeuAc as a substrate at a pH of 5.6. Values are the mean ± SD of three independent experiments.

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