ZFIN Image: Simonian et al., 2023, Fig. 6

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Figure Caption

Fig. 6 mthfr deficiency in zebrafish reduces folates and alters 1-carbon cycle methyl metabolites. Relative concentrations of whole-body (A–D) folates and (E–L) related 1-carbon metabolites in zebrafish larvae at 5 dpf. Comparisons were conducted between cas9 versus 4gRNA mthfr crispants (4gRNA) or age-matched WT controls versus mthfr −/− mutants (mthfr −/−, HSC194). Values are mean ± SEM, n = 3–4 biological replicates of pooled zebrafish/group. Each pool was composed of n = 40 larvae for folates and n = 20 larvae for methyl metabolites for cas9 versus 4gRNA mthfr crispants and n = 50 larvae for folates and n = 30 larvae for methyl metabolites for WT versus mthfr −/− mutants. No differences in pooled weight were observed. Analyzed by Student’s t-test stratified by mutagenesis approach. Significant at ^*P < 0.05, ^*^*P < 0.01, ^*^*^*P < 0.0001. Abbreviations: tHcy, total homocysteine; 5,10-CH-THF, 5,10-methenyltetrahydrofolate; 5,10-CH2-THF, 5,10-methylenetetrahydrofolate.

Acknowledgments

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