Fig. 4

Fig. 4

Fig. 4. OPLS–DA. (A–D) Score plot and loading scatter plot of positive and negative modes in reference to normalized data of the zebrafish larvae exposed to 100 nM HgCl₂ compared to control. (E) Hierarchical clustering using log₂ fold change value generate heatmap of the top 25 downregulated (blue) and upregulated (red) metabolites from the positive and negative ion modes after embryonic exposure to HgCl₂ compared to control (F) Targeted enrichment analysis obtained from –log of p–values and pathway impact values via the topology analysis lists potential signaling pathways dysregulated (yellow < orange < red – increasing statistical significance, circle diameter – pathway impact) after embryonic exposure to HgCl₂.