ZFIN Image: Das et al., 2023, Fig. 7

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Figure Caption

Fig. 7

Synergistic effect of NGP ASO and Taxol on the stemness property of MCF7 cells

(A) Expression of Oct4, p-STAT3, and FAK detected through flow cytometry analysis in Taxol-, NGP ASO-, NGP ASO + Taxol-, NP ASO-, and NP ASO + Taxol-treated MCF7 cells depicted through histogram (A1) and bar diagrams (A2). (B) Immunofluorescence and confocal microscopic analysis of co-localization of NANOG (green fluorescence) and Oct4 (red fluorescence) in control and other experimental types of treated cells (B1). Nuclei were stained with DAPI (blue fluorescence). Bar diagrams representing relative fluorescence intensity of NANOG (B2) and Oct4 (B3). (C) Confocal images (C1) after immunofluorescence of FAK (red fluorescence) in control and all treatment groups after 36 h with relative fluorescence intensity of FAK. Quantification of NANOG, Oct4, and FAK intensity per nucleus was calculated from confocal images of 20?25 cells each. Data presented as mean � SEM. ?p < 0.05.

Acknowledgments

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