ZFIN Image: Das et al., 2023, Fig. 1

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Figure Caption

Fig. 1

Chemical structures of GMO-PMO, MTT assay and dose dependent Gli1 gene silencing

(A) Chemical structures of (a) PMO, PPMO, and vivo PMO; (b) DNG; (c) GMO; (d) GMO-PMO; and (e) PMO-GMO chimeras. (B) Cell viability assay by MTT in Shh Light2 cells after treatment with different doses of GGP Scr MM. (C) Western blot analysis of Gli1 in ShhLight2 cells with different doses of multi-mutated (GGP Scr MM) and Gli1 antisense GMO-PMO (GGP ASO). (D) Comparison of protein repression potential of GGP Scr MM and GGP Scr SM; western blot analysis of the expression of Gli1- and Gli1-related proteins with GGP SCR SM (0.75 ?M), GGP SCR MM (0.75 ?M), GGP Scr ASO (0.75 ?M), GP ASO (0.75 ?M), and cyclopamine after 36 h of treatment. The densitometric analysis has been provided along with (E). (F) Western blot analysis of Gli1 at 0.75?M dose of GPG ASO and GGP ASO along with its densitometric analysis. Data presented as mean � SEM. ?p < 0.05.

Acknowledgments

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