Fig. 1

Biodistribution of PAP3 liposomes within zebrafish embryos (78 hpf). a) Schematic zebrafish larvae in lateral (whole-body) view, showing the site of microinjection and key cranial vessels. Fluorescently labeled liposomes are imaged with confocal microscopy after 60–90 min. The vasculature is as follows, liposome targeted bECs in yellow and systemic endothelium in dark gray, scavenger endothelial cells (SECs) in cyan, at ≈78 h post-fertilization (hpf). b) Biodistribution (10x magnification, lateral view) of PAP3 liposomes within a Tg(kdrl:GPF) zebrafish embryo at 1.5 h post-injection (hpi). c) Chemical structure of lipids used in the equimolar mixture for the formulation of PAP3 liposomes, DOaG, and DSPC lipids. d) Zoom of the cranial region in lateral view. bECs, brain endothelial cells; CtAs, central arteries; DLV, dorsal longitudinal vein; DMJ, dorsal midline junction; MCeV, middle cerebral vein; MsA, mesencephalic artery; MsV, mesencephalic vein; PMBC, primordial midbrain channel; PHBC, primordial hindbrain channel; SECs, scavenging endothelial cells. Liposomes formulated by extrusion (5 mM, 0.2% mol DOPE-LR). Scale bars: 200 µm (lateral view) and 100 µm (zoom).