ZFIN Image: Weinschutz Mendes et al., 2023, Fig. 7

Image

Figure Caption

Fig. 7 Dopaminergic and microglial phenotypes in scn1lab and dyrk1a mutants

(A) Hypothesis-driven GSEA of DE genes (p < 0.1 and fold-change >1.5) in scn1lab^?44/?44 and dyrk1aa^?77/?77dyrk1ab^?8/?8 mutants using cell-type-specific datasets.⁵⁸^,⁵⁹^,⁶⁰ Cell type markers enriched in upregulated (red) or downregulated (blue) genes are shown. Bubbles are shown only for cell type markers with significant enrichment (p < 0.05). The color intensity and size of each bubble represent the odds ratio. p-values calculated using Fisher?s exact test are shown in each bubble. For the complete GSEA in homozygotes and heterozygotes, see Figure S7A.

(B) Phospho-histone H3 (pH3, green) and acetylated tubulin (AcTub, magenta) immunostained whole brains of scn1lab^?44/?44 and dyrk1aa^?77/?77dyrk1ab^?8/?8 versus wild-type fish at 3 dpf. Dorsal views. FB, forebrain; OT, optic tectum; MHB, midbrain-hindbrain boundary. Scale bar, 50 ?m. For quantification by brain region, see Figures S7B?S7E.

(C and D) Total number of pH3+ cells in scn1lab^?44/?44 (n = 18) versus scn1lab^+/+ (n = 16) (C) and dyrk1aa^?77/?77dyrk1ab^?8/?8 (n = 14) versus wild-type fish (n = 19) (D). ^????p < 0.0001, one-way ANOVA.

(E) Tyrosine hydroxylase (TH, green) and acetylated tubulin (AcTub, magenta) immunostained whole brains of wild-type, scn1lab^?44/?44, and dyrk1aa^?77/?77dyrk1ab^?8/?8 fish at 4 dpf. Scale bar, 50 ?m. Ventral views. FB, forebrain; PT, posterior tuberculum.

(F and G) Total number of TH+ cells in scn1lab^?44/?44 (n = 20) versus scn1lab^+/+ (n = 19) in the forebrain (F) and posterior tuberculum (G) (boxes in [E]). ^????p < 0.0001, one-way ANOVA. For heterozygous phenotypes, see Figures S7F?S7G.

(H) Neutral red staining of live scn1lab^?44/?44 and dyrk1aa^?77/?77dyrk1ab^?8/?8 versus wild-type fish at 4 dpf. Scale bar, 0.1 mm.

(I and J) Total number of neutral red+ cells in scn1lab^?44/?44 (n = 17) versus scn1lab^+/+ (n = 20) (I) and dyrk1aa^?77/?77dyrk1ab^?8/?8 (n = 16) versus wild-type fish (n = 15) (J). ^????p < 0.0001, ^?p < 0.05, one-way ANOVA.

(K) 4C4 (green) and acetylated tubulin (AcTub, magenta) immunostaining of whole brains of dyrk1aa^?77/?77dyrk1ab^?8/?8 and wild-type fish at 4 dpf. Scale bar, 50 ?m. Dorsal views. FB, forebrain; OT, optic tectum; MHB, midbrain-hindbrain boundary.

(L and M) Total number of 4C4⁺ cells in dyrk1aa^?77/?77dyrk1ab^?8/?8 (n = 13) versus wild-type (n = 10) in whole brain (L) and forebrain (M). ^????p < 0.0001, ^??p < 0.01, one-way ANOVA.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.