Fig. 6
ChA induces lipid accumulation in the vasculature and is toxic to zebrafish larvae. Five days postfertilization, zebrafish larvae were fed for 10 days with normal (in gray), FC-enriched (in blue), or ChA-enriched (in red) diets. A: Confocal z-projection images of fluorescent lipid deposits (in red, indicated by the arrows) of caudal vein of AB larvae. For visualization of lipid structures, diets (normal, 2 or 4% FC-enriched, 3 or 6% ChA-enriched food) were supplemented with 10 μg/g of a red fluorescent CE. Scale bars represent 20 μm. B: Quantification of total lipid structure area in the zebrafish caudal vein. Fluorescent images of at least 10 larvae were quantified per condition. The results are shown as mean ± SEM; ∗∗∗