Figure 4

Figure 4

Zebrafish sirt7 suppresses hypoxia-responsive gene expression.A, Western blot analysis of indicated protein levels in EPC cells transfected empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 (Myc-sirt7). B, luciferase activity of hypoxia-responsive element (HRE)-luciferase reporter in EPC cells transfected with empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 (Myc-sirt7) under normoxia (Nor) or hypoxia (Hyp). C, Western blot analysis of indicated protein levels in ZFL cells transfected empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 (Myc-sirt7). D, quantitative real-time PCR (qPCR) analysis of phd3 in ZFL cells transfected with empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 (Myc-sirt7) under normoxia (Nor) or hypoxia (Hyp). E, qPCR analysis of vegfaa in ZFL cells transfected with empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 (Myc-sirt7) under normoxia (Nor) or hypoxia (Hyp). F, qPCR analysis of ldha in ZFL cells transfected with empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 (Myc-sirt7) under normoxia (Nor) or hypoxia (Hyp). G, qPCR analysis of cited2 in ZFL cells transfected with empty vector control (Myc empty) or Myc-tagged zebrafish sirt7 under normoxia (Nor) or hypoxia (Hyp). p Values were calculated by two-way ANOVA analysis (B, D, E, F, and G); ∗∗∗∗p < 0.0001; data based on one representative experiment performed in three biological replicates from at least three independent experiments (mean ± SD).