Fig 2

Fig 2 Tp induces microglial apoptosis and activation in vitro.

The percentage of apoptotic cells was significantly increased in HMC3 cells treated with Tp compared to untreated HMC3 cells, and the percentage of normal cells was significantly decreased after Tp infection in a dose-dependent manner (MOI of 10:1, 50:1, 100:1 or 150:1). (A) HMC3 cells were treated with Tp (MOI of 10:1, 50:1, 100:1, or 150:1) for 24 h. The percentages of apoptotic cells and living cells were analysed by flow cytometry. (B) Apoptosis assay of HMC3 cells after Tp treatment at an MOI of 50:1 for 24 h by TUNEL staining. Scale bar  =  100 μm. (C) HMC3 cells were treated with Tp (MOI of 50:1) for 24 h and immunostained for IBA. Scale bar  =  100 μm. (D) HMC3 cells were treated with Tp (MOI of 50:1) for 24 h, and flow cytometry was used to analyse MHC-II expression. The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.