Fig. 7

Expression of cytokine genes, tnfα, il1β, il8, il4, il6, and il10, in zebrafish guts. Abbreviations: Control refers to the non-supplemented TNBS-challenged group; Ns-L, 7.5% of non-starved L. incisa P127 biomass; Ns-H, 15% of non-starved L. incisa P127 biomass; St-L, 7.5% of N-starved L. incisa P127 biomass; Ns-H, 15% of N-starved biomass; DGLA, commercial DGLA ethyl ester. Values in box plots show relative gene expression levels normalized to the geomean of three housekeeping genes. n = 4 replicates; each replicate is a pool of five fish from a single replicate aquarium. Asterisks denote significant differences as compared to the control, as follows: * before challenge (healthy); ** after TNBS challenge. Significant differences within treatment groups of healthy (before challenge) and TNBS-challenged fish are indicated by a bar, with the p value in brackets.