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Fig. 7
Effects of 5-HT7R agonist (LP-12) only or combined with its antagonist (SB269970) on melanogenesis, dendrite, and migration in normal human melanocytes (NHMCs). (A) 5-HT7R or DAPI respectively were stained with green or blue. (B) The effects of LP-12 and SB269970 on melanin contents in the NHMCs. Each value represents the mean ± SEM (n = 3). **p < .01, compared with the Ctrl, ###p < .001, compared with the LP-12. (C,D) LP-12 (200 nM) and SB269970 (100 ?M) respectively regulated dendrite and migration in the NHMCs. Bar = 25 ?m. (E) Left, the expressions of 5-HT7 receptor, MITF, Tyr, and DCT were upregulated after the 5-HT or LP-12 were treated in the NHMCs, and SB269970 could inhibit it. It also could initiate the downstream ERK1/2 and JNK MAPKs signaling pathway through the 5-HT7R activation, thereby promoting the melanin synthesis process. Right, LP-12 respectively promotes downstream Rab27a and RhoA or PI3K/AKT pathway and FAK phosphorylation to improve the functions of dendrite growth and migration in NHMCs. Representative image from three independent experiments is shown.
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