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Fig. 6
Effects of 5-HT7R selective agonist LP-12 on melanosome maturation or quantities and 5-HT7R Silencing Test and IP on melanogenesis process in B16F10 cells. (A) LP-12 (200 nM) treated with B16F10 cells for 72 h, compared with Ctrl, could cause cellular hyper-pigmentation via promoting melanosomes maturation and quantities observed by transmission electron microscopy (Tecnai G2 Spirit BioTWIN, 2550× or 4200×). Each value represents the mean ± SEM (n = 3). (B) Expression of 5-HT7R gene silencing fragment protein. (C) Left, effect of silencing 5-HT7R gene on melanin contents in B16F10 cells. *p < .05, compared with LP-12 + si-Ctrl group. Right, observation of melanin granules by silencing 5-HT7R gene. Bar = 50 ?m. (D) Observation of dendritic morphology by silencing 5-HT7R gene. Bar = 50 ?m. Original magnification was 400×. (E) Effect of silencing 5-HT7R gene on interaction of PKA-RII-? and PKA C-? protein in vitro. Representative image from three independent experiments is shown.
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