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Fig. 2
Effects of 5-HT7R agonist (LP-12) on CRS-induced pigment loss in C57BL/6 mice. (A) LP-12 increased pigment accumulation in C57BL/6 mice compared WITH CUMS (n = 5). (B) Serum 5-HT, NE, and CORT levels were measured in various mouse groups **p < .01, ***p < .001, compared with the Ctrl. #p < .05, ###p < .001, compared with the CRS. (C) Expressions of CD8a+, IFN-?, and CD8a+ + IFN-? were severally tested in the CRS-induced mice? spleen cells of different groups (n = 3). *p < .01, **p < .01, ***p < .001, compared with the Ctrl. ##p < .01, compared with the CRS. (D) Left, immunofluorescent staining of sympathetic nerves or ganglia (green) for tyrosine hydroxylase (TH) in the skin tissues (n = 3 mice for each condition). Middle, the mice stimulated with CRS were respectively marked with mostly white hairs (green box). Right, Fluorescence intensity of sympathetic nerve was statistically evaluated by Image J. Bar = 25 ?m. Original magnification was 400×. (E) Left, immunofluorescent staining of melanosomes (red) for HMB45 in the skin tissues (n = 3). Middle, the mice stimulated with CRS were respectively marked with mostly white hairs (red box). Right, Fluorescence intensity of HMB45 was statistically evaluated by Image J. Original magnification was 400×. Bar = 25 ?m. Representative images from three mice were shown.
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