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Fig. 5
Expression of nod1 and nod2, pparĪ³, and rela (p65 subunit of NFkB) genes in the guts of zebrafish fed with the different experimental diets. Abbreviations: Control refers to the non-supplemented TNBS-challenged group; Ns-L, 7.5% of non-starved L. incisa P127 biomass; Ns-H, 15% of non-starved L. incisa P127 biomass; St-L, 7.5% of N-starved L. incisa P127 biomass; Ns-H, 15% of N-starved biomass; DGLA, commercial DGLA ethyl ester. Values in box plots show relative gene expression levels normalized to the geomean of three housekeeping genes. n = 4 replicates; each replicate is a pool of five fish from a single replicate aquarium. Asterisks denote significant differences as compared to the control, as follows: * before induction (healthy); ** 24 h after TNBS application. Significant differences within treatment group of healthy (before challenge) and TNBS-challenged fish are indicated by a bar, with the p value in brackets.
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