Figure 1-supplement 2.

Figure 1-supplement 2. dmist^vir/vir fish are hyperactive and have normal circadian rhythms.

(A) Free-running circadian period length of the locomotor activity of larvae from a dmist^vir/+ in-cross following the transition at 5 dpf from a 14 hr:10 hr light:dark cycle to constant dark conditions. The data is quantified for 48 hr after the shift to darkness and shows no difference in period between dmist^vir/vir larvae and their sibling controls. Data is from three independent experiments. p>0.05, one-way ANOVA, Tukey’s post hoc test. (B, C) Representative mean ± SEM sleep (B) and waking activity (C) traces of animals used to calculate circadian period length in (A). Light and dark grey blocks show subjective day and night, respectively. (D) RT-qPCR time-course before (light) and after (grey) transfer into constant dark demonstrates that dmist mRNA levels do not oscillate with a circadian period, unlike per1 mRNA which does. n = 3 replicates per timepoint. Expression is normalised to circadian time 3. Data are mean ± SEM.