The klf1-klf17 double mutant exhibited impairment of primitive erythropoiesis. (A–D) Live embryos at 48 hpf. The red colour of blood-circulating erythroid cells (arrowheads) was faint in the klf1-klf17 mutant (klf1-/-klf17-/-) (D) compared to those in the wild-type (klf1+/-klf17+/+) (A), klf1 mutant (klf1-/-klf17+/-) (B) and klf17 mutant (klf1+/+klf17-/-) (C). (E–H) Detection of haemoglobin (arrowheads) at 48 hpf. Haemoglobin production was examined by o-dianisidine staining. Haemoglobin production in the yolk was reduced in the klf1-klf17 mutant (H) compared to the wild-type (E), klf1 mutant (F) and klf17 mutant (G). Genotyping was performed by genomic PCR using locus-specific primers. Scale bar, 200 μm (A, E).
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