Fig. 9

Fig. 9 Spatial configuration of segregated domains depends on actomyosin contractility regulation.

a Time-dependent increase in mean segregated cell cluster sizes in aggregates (n = 16) forming in mixed suspensions of normal zebrafish ectoderm cells (ectoderm-ctrl) and mesoderm cells. b Representative 3D reconstruction (left panel) and simultaneous epifluorescent (right panel, bottom view) images from time-lapse videos of segregating clusters of ectoderm-ctrl (red) and mesoderm (green) cells after 12 h of segregation, see Supplementary Movie 13. c Time-dependent growth of segregated cell cluster sizes in aggregates (n = 15) of mixed ectoderm cells overexpressing constitutively active ROCK (ectoderm-caROCK) and mesoderm cells. d Representative 3D reconstruction (left) and epifluorescent (right, bottom view) images of segregating clusters of ectoderm-caROCK (red) and mesoderm (green) cells after 12 h of segregation, see Supplementary Movie 14. Note the inverted spatial configuration of segregated domains here, compared to panel (b). Scale bar: 100 µm in (b, d). Error stripes represent SEM in (a, c). Also see Supplementary Fig. 5.