Fig. 6

Fig. 6

Fig. 6. Transcriptional impact of her3 mutation status. (A) Schematic of RNA collection. Embryos from wildtype WIK in-cross and her3^nch1 in-cross were collected, with a subset of WIK embryos injected with either her3-MO or mismatch-MO. At 24hpf, RNA was isolated from 3 groups of 25 embryos each per condition, and again at 72hpf. RNA was then sent for sequencing. (B) PCA of all samples, excluding MO samples. (C–D) PCA of her3^nch1 and WIK 24hpf samples (C) and 72hpf samples (D). (E) Heatmap clustering of all differentially expressed genes from her3^nch1 and WIK samples. (F–G) Volcano plots of differentially expressed genes in her3^nch1 compared to WIK 24hpf samples (F) and 72hpf samples (G). Differential expression was considered significant with an FDR ≤0.1 and absolute value of logFC ≥1.5. Top annotated up- and downregulated genes are labeled. (H) Tile chart showing pairwise comparisons of differentially expressed genes. Numbers are displayed as downregulated/upregulated of the groups along the x-axis.