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Figure 1

ID: ZDB-IMAGE-230731-59
Source: Figures for Wang et al., 2023

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Figure 1

Construction of heritable hoxa1a mutants by CRISPR/Cas9. (A) Graphic illustration of CRISPR targeting the first exon in hoxa1a locus. (B) Determination of mutation efficiency by T7 Endonuclease I assay. Five founders with three replicates were examined. (C) Positive mutants in F1 offspring. (D) 3D structure prediction of normal 329aa hoxa1a protein and 41aa truncated mutant. (E) Sanger sequencing results of hoxa1a homozygous and heterozygous mutants.

Acknowledgments

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