Figure 9

Figure 9

Tumor-induced immunosuppression is reduced with the Mgst1 KD strategy in C57BL/6 mice.A, frequency of intratumor lymphocytes analyzed by flow cytometry (n = 5/group). B, tumor-infiltrated lymphocytes retrieved from the tumor-bearing mice were used to determine either surface expression of PD1, TIM3, KLRG1, and LAG3 (n = 4/group) or (C) were activated in vitro with phorbol 12-myristate 13-acetate and ionomycin (500 ng/ml) and assessed for intracellular IFNγ, TNFα, and Granzyme B production using flow cytometry (n = 5/group). For (B), the adjacent bar graphs represent the cumulative data of mean fluorescence intensity of the cell surface expression of PD1, TIM3, KLRG1, and LAG3 on the tumor-infiltrated CD8⁺ T cells. For (C), the adjacent bar graphs represent the cumulative data of frequency of cytokine-secreting cells. ∗, ∗∗, and ∗∗∗ indicate p < 0.05, p < 0.005, and p < 0.0005, respectively. KD, knockdown; MGST, microsomal glutathione transferase.