Figure 2

Figure 2

Expression of early and late neural crest markers by in situ hybridization: (A,B) Similar sox10 expression at neural plate border in WT (A) and apc^mcr/mcr (B) embryos at 12 hpf. Genotypes in (A) (n = 30 WT or apc^mcr/+) and (B) (n = 7 apc^mcr/mcr) were defined by genomic PCR on individual larvae. (C,D) In WT embryos at 55 hpf (C), gsc is expressed in the mandibular (m, including Meckel’s cartilage), hyoid (h), and more posterior branchial arches, as well as two symmetric anterior domains, as described previously [44]. In apc^mcr/mcr larvae (D), gsc is undetectable in the mandibular and hyoid arches. (For WT, n = 15, and for apc^mcr/mcr, n = 7, based on morphological phenotype). All scale bars are 200 µm.