Figure 6

Figure 6

mcm10-deficient embryos accumulate DNA damages in nascent HSCs

(A) Western blot to quantify γH2A.X in control or mcm10 morphants.

(B) Statistical analysis of the ratio γH2A.X/actin was completed using an unpaired two-tailed t test. ^∗p < 0.01 (three independent experiments, with >30 embryos pooled per condition, per experiment).

(C) Anti-GFP and γH2A.X stainings performed on 32-hpf cmyb:GFP embryos, either injected with control or mcm10 morpholinos.

(D) Quantification of the number of double-positive cells in the aorta floor of control and mcm10 morphants. Center values denote the mean, error values denote SEM, and statistical analysis was completed using an unpaired two-tailed t test. ^∗∗∗p < 0.001 (n = number of total embryos from three independent experiments). Scale bar: 50 μm (C).