Figure 4

Figure 4

mcm10 overexpression increases the expression HSC marker

(A) runx1 expression in 28-hpf embryos, either non-injected or injected with mcm10 full-length mRNA.

(B) Statistical analysis was completed using Fisher’s exact test. ^∗∗∗p < 0.001 (n = number of total embryos from three independent experiments).

(C) WISH against cmyb at 36 hpf in embryos, either non-injected or injected with mcm10 full-length mRNA.

(D) Statistical analysis was completed using Fisher’s exact test. ^∗∗p < 0.01 (n = number of total embryos from three independent experiments).

(E) cmyb expression in 48-hpf embryos, either non-injected or injected with mcm10 full-length mRNA.

(F) Quantification of the number of cmyb-positive cells in the CHT per embryo. Statistical analysis was completed using an unpaired two-tailed t test. ^∗∗p < 0.01 (n = number of total embryos from three independent experiments).

(G) WISH against cmyb in 5-dpf embryos, either non-injected or injected with mcm10 full-length mRNA.

(H) Statistical analysis was completed using Fishers exact test. ^∗∗∗p < 0.001.

(I) WISH against rag1 at 5 dpf in embryos, either non-injected or injected with mcm10 full-length mRNA.

(J) The area of the thymus was measured for each embryo. Statistical analysis was completed using an unpaired two-tailed t test. ^∗∗p < 0.01 (n = number of total embryos from three independent experiments). Scale bars: 100 μm (A, C, E, G, and I).