Figure 3

Figure 3

mcm10 deficiency induces apoptosis in developing HSCs

(A) Fluorescence imaging of the dorsal aorta in flk1:mCherry;cmyb:GFP embryos injected with control or mcm10-MOs.

(B) Quantification of HSCs. Statistical analysis: unpaired two-tailed t test, ^∗∗∗p < 0.001 (n = number of total embryos from three independent experiments).

(C) Anti-GFP and TUNEL stainings of cmyb:GFP embryos at 32 hpf, after injection of either control or mcm10 morpholinos (mcm10-MO).

(D) Quantification of the number of GFP+ and TUNEL+ cells in control and mcm10-morphants. Center values denote the mean, error values denote SEM, and statistical analysis was completed using an unpaired two-tailed t test. ^∗∗∗p < 0.001 (n = number of total embryos from three independent experiments). Scale bars: 50 μm (A), 100 μm (C).