Figure 2

Figure 2

The loss of mcm10 affects HSCs emergence

(A) WISH for runx1 expression at 28 hpf in mcm10^−/− embryos and their siblings and after mcm10 knockdown by morpholino injection.

(B) WISH for cmyb expression along the aorta at 36 hpf in control and mcm10-morphants.

(C) Statistical analysis was completed using Fisher’s exact test, ^∗∗∗∗p < 0.0001 (n = number of total embryos from three independent experiments).

(D) WISH for cmyb expression in the CHT at 48 hpf in control and mcm10 morphants.

(E) Statistical analysis was completed using unpaired two-tailed t test, ^∗∗∗p < 0.001 (n = number of total embryos from three independent experiments). Scale bars: 100 μm (A, B, and D).