Fig. 6

In vivo antitumor effects of compound 1b in zebrafish xenografts. CM-DiI-stained A549 cells were transplanted into two dpf zebrafish embryos via microinjection. (A) Representative images of the relative intensity and distribution of the red fluorescence using confocal microscopy after testing the concentration of compound 1b administration (1, 3, and 9 μM) for 48 h. (B) Histogram of fluorescence intensities and quantification of the fluorescent area of the compound 1b-treated tumor xenografts. Results are presented as the means ± SD (n = 3). ** p < 0.01, *** p < 0.001 vs. the control group. ^# p < 0.05, ^## p < 0.01 vs. the positive control etoposide.