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Fig. 5 gad67 and nkx1.2lb expression required transcription factor Tal2. (A-B′) Embryos injected with a mismatch control morpholino (A and B) or a morpholino directly targeted tal2 mRNA (A′ and B′). gad67 expressing cells are abolished in the nMLF in morphant (cycle with dash line), whereas gad67-positive cells in the forebrain are present (A′). tal2 knockdown completely eliminates nkx1.2lb expressing cells in the diencephalon and nMLF/nPC, whereas nkx1.2lb expressing cells are unaffected in the hindbrain (B′). (C and C′) Embryos injected with a mismatch control morpholino (C) or a splice morpholino directly targeted exon-intron boundary of nkx1.2lb (C′). The expression of gad67 is unaffected in the nMLF. All embryos' eyes were removed by dissection. Dorsal is up; rostral, to the left. DMB, diencephalon midbrain boundary; nMLF, nucleus of the medial longitudinal fasciculus; PT, posterior tuberculum; Tel, telencephalon; PTh, prethalamus. Scale bar: 50 μm