ZFIN Image: Wang et al., 2022, Fig. 6

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Figure Caption

Fig. 6 Morpholinos used in this study have high efficiency and specificity. (A-C′) Embryos injected with a mismatch control morpholino (A-C) or a morpholino directly targeted gata2a, gata3, and tal2 mRNA (A′, B′, C′). Injection of gata2a and tal2 morpholinos abolishes gad67 expressing in the KA″ cells. The expression of gad67 in the KA′ cells is eliminated by knockdown of gata3. (D) Injection of splice morpholino directly targeted exon-intron boundary of nkx1.2lb generates a smaller RT-PCR DNA fragment than that of the control. (E and F) DNA sequencing reveals a generation of 137 bp deletion in embryos injected splice morpholino against nkx1.2lb (E, marked by dotted line), in comparison with control (F). Dorsal is up; rostral, to the left. Scale bar: 50 μm

Acknowledgments

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