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Fig. 1 Nodal-related factors and shh signaling are required for gad67, gata2a, gata3, tal1, tal2, and nkx1.2lb expressing in the diencephalon and ventral midbrain. (A-F?) Heads of wild-type (A-F) and matching homozygous cyclops mutants (A?-F?) and smu mutants (A?-F?) of 30 hpf zebrafish embryos hybridized to gad67 (A-A?), gata2a (B-B?), gata3 (C-C?), tal1 (D-D?), tal2 (E-E?), and nkx1.2lb (F-F?) probes. The expression of gad67 (A?) in the diencephalon, and the nucleus of the medial longitudinal fasciculus (nMLF) is abolished in the cyc?/? mutant, while smu?/? embryo (A?) lacks gad67 expressing cells in the nMLF (within the circle). The cyc?/? and smu?/? embryos lack the expressions of gata2a, gata3, tal1, tal2, and nkx1.2lb in the presumptive nMLF, and disturb their expression domains in the diencephalon. All embryos' eyes were removed by dissection. Dorsal is up; rostral, to the left. DMB, diencephalon midbrain boundary; HB, hindbrain; MHB, midbrain hindbrain boundary; nMLF, nucleus of the medial longitudinal fasciculus; PT, posterior tuberculum; PTh, prethalamus; Tec, tectum; Teg, tegmentum; Tel, telencephalon; Th, thalamus. Scale bar: 50 ?m