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Fig. 8
Effects of ES24 on cell membrane function in E. coli. (A) DiSC(3)5 measurements of E. coli MC4100 carrying pABCON2-fhuA ΔC/Δ4L treated with ES24 or NFT. Polymyxin B (PolB) was used as positive control. p-Values were calculated using a heteroscedastic, two-tailed t test. *p < 0.05, **p < 0.01, ***p < 0.001. (B) MinD oscillation in E. coli RC1 carrying pFX9 treated with ES24 or NFT. The proton ionophore CCCP, which is known to abolish MinD oscillation due to membrane depolarization,40 was used as positive control. (C) Quantification of FtsZ delocalization. Cells displaying an entirely cytosolic GFP signal, GFP clusters in the cell membrane, double bands, or spirals of FtsZ were counted as delocalized. Cells displaying single FtsZ bands, two opposing foci, or one focus at midcell (indicative of a closing septum) were counted as normal.