Figure 2

Figure 2

Larval inflammation/immune pathways are upregulated in anxiety and downregulated in obesity. (A) PANTHER Overrepresentation Test identified “CCKR—gastrin- and cholecystokinin-mediated regulation of cellular processes signaling map”, “inflammation mediated by chemokine and cytokine signaling pathway”, and “interleukin signaling pathway”. Reference genes were all those included in the Danio rerio database, Fisher’s exact test, correction by FDR < 0.05. (B) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) results of the anxiety and obesity models are presented as graphs, with RNA-seq results below each graph, n= 6–8 replicates (10–30 larvae/replicate), 2–3 separate experiments, Student’s t-test. Reference genes are the average of eef1a1l1 and actb2. *—p < 0.05, **—p < 0.01, ***—p < 0.001.