Fig. 6

Fig. 6

Endothelial Flvcr1a targeting increases tumor vessel permeability and hypoxia. A–D DCE-MRI analysis to evaluate tumor vessel permeability in LLC xenografts developed in Flvcr1a^fl/fl;Cdh5-Cre^ERT2 and Flvcr1a^fl/fl mice. The B enhancement curve, C K^trans constant, and D k^ep constant are shown. E Immunofluorescence analysis on Flvcr1a^fl/fl and Flvcr1a^fl/fl;Cdh5-Cre^ERT2 tumor sections stained with hypoxia marker pimonidazole (red) and DAPI (blu). Scale bar: 200 µm.; ECs endothelial cells, PIMO pimonidazole, DCE-MRI Dynamic Contrast-Enhanced Magnetic Resonance Imaging. Data are representative of at least 3 independent experiments and are expressed as mean ± SEM. **p < 0.01; For statistical analyses, an unpaired Student’s t test was used