Fig. 1

Fig. 1 STAT3 regulates a subset of hypoxia-dependent genes.

A–D Transcriptome analysis by RNA-seq. A: Genes that were differentially expressed (log2[fold change (FC)] > + 0.75 or <−0.75; q-value < 0.01, Benjamini–Hochberg adjustment, as indicated by dashed lines) between Stat3^+/+ cells in normoxia and Stat3^+/+ cells in hypoxia; n = 4 biological replicates. Expression levels of genes that were down- (B) and upregulated (C) in Stat3^+/+ in hypoxia relative to Stat3^+/+ cells in normoxia. Each boxplot shows the 1st, 2nd and 3rd quartiles; the whiskers show the minimum and maximum values. D heatmap of RNAseq data reporting the expression of genes that are affected by hypoxia in Stat3^+/+ cells compared to Stat3^+/+ normoxic cells, but that require STAT3 to be properly altered in their expression. E expression level of Vegfa, Hk1, Hk2, Pfkp and Hilpda in normoxic and hypoxic Stat3^+/+ and Stat3^−/− mESCs taken from RNAseq data. F Gene expression analysis by RT–qPCR of Stat3^+/+ and Stat3^−/− cells treated in normoxic and hypoxic mESCs of the same genes shown for the RNAseq. Mean ± SEM of n = 4 experiments, with each replica shown as a dot.